===TCGASpliceSeq可变剪切分析===


[[https://ngdc.cncb.ac.cn/databasecommons/database/id/1632]]


[[https://bioinformatics.mdanderson.org/TCGASpliceSeq/]]

软件安装网页介绍，https://bioinformatics.mdanderson.org/public-software/spliceseq/installation/

mysql 服务自己安装未成功，使用比较转录组的流程。

构建数据库过程如下：
  /PUBLIC/software/public/System/mysql-5.1.62/bin/mysql -uroot -h 10.1.50.254 --port 3310 -pnovorna #进入交互页面
  mysql>GRANT SELECT ON SpliceGraph.* TO 'sguser'@'localhost' IDENTIFIED BY 'sgpass';
  mysql>GRANT SELECT ON SpliceGraph.* TO 'sguser'@'%' IDENTIFIED BY 'sgpass';
  mysql>GRANT ALL ON SpliceGraph.* TO 'sgload'@'localhost' IDENTIFIED BY 'sg4ld!';
  mysql>GRANT ALL ON SpliceGraph.* TO 'sgload'@'%' IDENTIFIED BY 'sg4ld!';
  mysql>exit;
  
  /PUBLIC/software/public/System/mysql-5.1.62/bin/mysql -uroot -h 10.1.50.254 --port 3310 -pnovorna < /TJPROJ6/RNA_SH/software/software/SpliceSeq/database/SpliceGraphDB.sql
  
分析参考路径 /TJPROJ6/RNA_SH/shouhou/pip_example/SpliceSeq/X101SC23034437-Z01-J081