报错内容

ERROR	2022-05-25 23:25:29	ReorderSam	No reference sequence dictionary found. Aborting.  You can create 
a sequence dictionary for the reference fasta using CreateSequenceDictionary.jar.

解决方法

用gatk建立序列字典
/TJPROJ2/GB/PUBLIC/software/GB_TR/mRNA/miniconda3/envs/SNP/bin/java  -jar 
/TJPROJ2/GB/PUBLIC/software/GB_TR/mRNA/miniconda3/envs/SNP/share/gatk4-4.1.1.0-0/gatk-package-4.1.1.0-local.jar 
CreateSequenceDictionary -R=genome.fa -O=genome.dict