软件路径:
/TJPROJ6/RNA_SH/personal_dir/zhangxin/miniconda/envs/python_3.7.0/bin/manorm
1.首先将比对之后的bam文件转化成bed格式
/PUBLIC/software/RNA/bedtools/bedtools-2.25.0/bin/bamToBed -i OG.uni.dedup.bam > OG.uni.dedup.bed
2.分析:
/TJPROJ6/RNA_SH/personal_dir/zhangxin/miniconda/envs/python_3.7.0/bin/manorm --p1 /TJPROJ13/GB_TR/PJ_AI/ChIPseq/gn_068/X101SC2212/X101SC22124752-Z01-J001_20230422092523/output/peak/OG_peaks.xls --p2 /TJPROJ13/GB_TR/PJ_AI/ChIPseq/gn_068/X101SC2212/X101SC22124752-Z01-J001_20230422092523/output/peak/CK_peaks.xls --pf macs2 --r1 /TJPROJ6/NC_BG_SH/shouhou/202305/X101SC22124752-Z01-J001/input/OG.uni.dedup.bed --r2 /TJPROJ6/NC_BG_SH/shouhou/202305/X101SC22124752-Z01-J001/input/CK.uni.dedup.bed --n1 OG --n2 CK --o /TJPROJ6/NC_BG_SH/shouhou/202305/X101SC22124752-Z01-J001/python3/OG_vs_CK
–p1,–p2 为macs2分析出来的peak文件; –pf 分析软件 ; –r1 ,–r1 为bed文件, –n1,–n1 比较组名字; –o 输出路径