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ciriquant

使用CIRIquant软件进行circRNA鉴定和定量

#~~~~
#运行脚本:
python /TJPROJ6/RNA_SH/personal_dir/sunjiaxue/scripts/get_CIRIquant.py \
  --rawdata ./rawdata \
  --sample Sample_1,Sample_2,Sample_3 \
  --ref_yml ./Homo_sapiens_Ensembl_110_ref.yml \
  --outfile ./tt

#~~~~
Homo_sapiens_Ensembl_110_ref.yml文件格式:
name: Homo_sapiens_Ensembl_110
tools:
  bwa: /TJPROJ6/RNA_SH/personal_dir/zhangxin/miniconda/envs/CIRIquant_v1.1.3/bin/bwa
  hisat2: /TJPROJ6/RNA_SH/personal_dir/zhangxin/miniconda/envs/CIRIquant_v1.1.3/bin/hisat2
  stringtie: /TJPROJ6/RNA_SH/personal_dir/zhangxin/miniconda/envs/CIRIquant_v1.1.3/bin/stringtie
  samtools: /TJPROJ6/RNA_SH/personal_dir/zhangxin/miniconda/envs/CIRIquant_v1.1.3/bin/samtools

reference:
  fasta: ./ref/Homo_sapiens_Ensembl_110.fa
  gtf: ./ref/Homo_sapiens_Ensembl_110.gtf
  bwa_index: ./ref/Homo_sapiens_Ensembl_110.fa
  hisat_index: ./ref/Homo_sapiens_Ensembl_110
ciriquant.txt · 最后更改: 2024/12/31 08:43 由 sunjiaxue