售后

湖南大学生物学院彭友松团队在生物信息学国际权威期刊《Briefings in Bioinformatics》（IF2021=14）在线发表题为“vsRNAfinder: a novel method for identifying high-confidence viral small RNAs from small RNA-Seq data”的研究论文，该研究开发了一种基于sRNA测序数据识别病毒sRNA的生物信息学工具vsRNAfinder，为深入挖掘病毒sRNA提供了帮助。

vsRNAfinder由四个模块组成，包括预处理模块（Preprocessing）、识别模块（Identification）、过滤和注释模块（Filtering and annotation）以及定量模块（Quantification），可在Github上获取（https://github.com/ZenaCai/vsRNAfinder）。该工具通过对病毒参考基因组上的覆盖度进行平滑化处理来降低峰值检测的噪声，进而增强峰值信号，便于峰值检测；同时为了得到高可信度的病毒sRNA，引入了泊松分布模型计算候选sRNA的统计学显著性，进一步提高所识别到的病毒sRNA的可信度。

https://github.com/ZenaCai/vsRNAfinder

http://bio.hnu.edu.cn/info/1286/7064.html

https://academic.oup.com/bib/article-lookup/doi/10.1093/bib/bbac496

第一步Preprocessing

export PATH=/TJPROJ6/RNA_SH/personal_dir/fengjie/SOFTWARE/CONDA/conda/envs/vsRNAfinder/bin/:$PATH

source /TJPROJ6/RNA_SH/personal_dir/fengjie/SOFTWARE/CONDA/conda/bin/activate vsRNAfinder

python /TJPROJ6/RNA_SH/personal_dir/fengjie/SOFTWARE/CONDA/conda/envs/vsRNAfinder/bin/Preprocessing.py \
	--cleanfq /TJPROJ6/RNA_SH/personal_dir/fengjie/Personal_analysis/vsRNAfinder/test/test.fa \
	--genome /TJPROJ6/RNA_SH/personal_dir/fengjie/Personal_analysis/vsRNAfinder/test/genome.fa \
	--outdir /TJPROJ6/RNA_SH/personal_dir/fengjie/Personal_analysis/vsRNAfinder/test \
	--prefix nc \

参考文献：

Zena Cai, Ping Fu, Ye Qiu, Aiping Wu, Gaihua Zhang, Yirong Wang, Taijiao Jiang, Xing-Yi Ge, Haizhen Zhu, Yousong Peng, vsRNAfinder: a novel method for identifying high-confidence viral small RNAs from small RNA-Seq data, Briefings in Bioinformatics, bbac496, https://doi.org/10.1093/bib/bbac496